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11th NanoBME Series Seminar
The endothelial cell glycocalyx: a dynamic participant in vascular wall biology
| Date | Monday 28 April 2008 15:00-16:30 |
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| Place | 21COE/REDEEM Lecture Hall, Biomedical Engineering 21COE/REDEEM Building (Aobayama Campus) |
| Outline |
(Professor of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology) 「The endothelial cell glycocalyx: a dynamic participant in vascular wall biology」 Recent research has identified the biological importance of the thin bounding layer called the glycocalyx that appears on the apical side of endothelial cells. It modulates the response of the cells to fluid flow in a number of important ways: changes in NO production; changes in crawling speed following flow onset; changes in cell division rate; and changes in cell alignment are among the most studied to date. With this motivation, we examine new methods to measure the mechanical properties of the layer. By using quantum dots attached to various participating proteins in the glycocalyx layer, we find that the layer is significantly deflected by the fluid shear force imposed by external flow at the level that the cells would experience in human arteries. The layer appears to have large differences between its properties near the membrane surface and near the external edge bounding the flow. Near the outer edge, the long heparin sulfate strands are highly elastic and recover their shape within a second. Motion near the membrane surface is highly restricted. The results indicate that the elastic properties of the glycocalyx vary significantly across the layer. |
| Contact | COE secretariat Tel：022-795-7005 E-mail: secretary@nanoBME.org |
